{"id":2594,"date":"2020-02-05T13:32:06","date_gmt":"2020-02-05T13:32:06","guid":{"rendered":"https:\/\/a031212db6.nxcli.net\/in-vitro-services\/mitochondria-related-assays\/"},"modified":"2023-11-29T19:01:10","modified_gmt":"2023-11-30T01:01:10","slug":"mitochondria-related-assays","status":"publish","type":"page","link":"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/in-vitro-services\/mitochondria-related-assays\/","title":{"rendered":"\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u95a2\u9023\u30a2\u30c3\u30bb\u30a4"},"content":{"rendered":"<section class=\"gb-container gb-container-d9f826a9\">\n<div class=\"gb-container gb-container-262196df\">\n<div class=\"gb-grid-wrapper gb-grid-wrapper-0b63e83a\">\n<div class=\"gb-grid-column gb-grid-column-c781dc1f\"><div class=\"gb-container gb-container-c781dc1f\">\n\n<p>A reduction in mitochondrial activity has been strongly linked with various neurodegenerative diseases. Moreover, several mutations have been characterized that induce age-related mitochondrial malfunction. QPS Austria provides cell culture models that address the effect of compounds on mitochondrial activity and mitochondria related cell death&nbsp;<em>in vitro<\/em>.<\/p>\n\n\n\n<p>Cell lines and primary neurons can be exposed to different toxins and examined for&nbsp;<strong>mitochondrial activity<\/strong>&nbsp;and&nbsp;<strong>mitochondrial membrane depolarization<\/strong>.<\/p>\n\n\n<div class=\"gb-container gb-container-9af486b0\">\n<div class=\"gb-container gb-container-e4f68970 gb-accordion\">\n<div class=\"gb-container gb-container-5eada5c9 gb-accordion__item\" data-transition=\"slide\">\n\n<button class=\"gb-button gb-button-e94e60dc gb-accordion__toggle\"><span class=\"gb-button-text\">\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u6d3b\u6027\uff08MitoTracker\uff09<\/span><span class=\"gb-icon\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon-open\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><\/span><\/button>\n\n\n<div class=\"gb-accordion__content\"><div class=\"gb-container gb-container-758dca46\">\n\n<p>Primary E18 rat hippocampal neurons were cultured on poly-lysine coated glass coverslips until DIV8. Next, neurons were treated with FCCP, a protonophore and uncoupler of oxidative phosphorylation in mitochondria, for a defined period of time. Labeling of neurons with MitoTracker Red indicates active mitochondria. After fixation, neurons were labelled for MAP2. The effect of compounds on active mitochondria in soma versus neurites can be evaluated in individual neurons using Image Pro Plus software.<\/p>\n\n\n\n<figure class=\"gb-block-image gb-block-image-951c5d07\"><img loading=\"lazy\" decoding=\"async\" width=\"902\" height=\"382\" class=\"gb-image gb-image-951c5d07\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure1.png\" alt=\"Mitochondria-related-assays-figure1\" title=\"Mitochondria-related-assays-figure1\" srcset=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure1.png 902w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure1-300x127.png 300w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure1-768x325.png 768w\" sizes=\"(max-width: 902px) 100vw, 902px\" \/><\/figure>\n\n\n\n<p><strong>Figure:<\/strong>&nbsp;Effect of FCCP on active mitochondria in primary hippocampal rat neurons. FCCP treatment significantly reduced MitoTracker Red labeling in somata and neurites of primary rat hippocampal neurons (see graph). Images indicate (left) control neurons and (right) neurons upon FCCP treatment. MitoTracker labeling is indicated in red, MAP2 labeling in green.<\/p>\n\n\n\n<p>Remark: This assay can also be performed with other mitochondria related toxins.<\/p>\n\n<\/div><\/div>\n<\/div>\n\n<div class=\"gb-container gb-container-e7f02fde gb-accordion__item\" data-transition=\"slide\">\n\n<button class=\"gb-button gb-button-d9dd69de gb-accordion__toggle\"><span class=\"gb-button-text\">\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u819c\u8131\u5206\u6975\uff08JC-1\uff09<\/span><span class=\"gb-icon\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon-open\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><\/span><\/button>\n\n\n<div class=\"gb-accordion__content\"><div class=\"gb-container gb-container-f56c19d6\">\n\n<p>Differentiated SH-SY5Y human neuroblastoma cells were lesioned with the Calcium ionophore ionomycin or with MPP+. Lesions were examined with JC-1 for mitochondrial membrane depolarization and with the MTT assay for cell viability in 96-well plates. JC-1 labelling was determined in a 96-well fluorescence plate reader with the appropriate filter settings. The ratio of red\/green fluorescence in control cells was set to 100%. Compounds can be examined for their ability to counteract mitochondrial membrane depolarization induced by selected lesion agents.<\/p>\n\n\n\n<figure class=\"gb-block-image gb-block-image-3554d0f9\"><img loading=\"lazy\" decoding=\"async\" width=\"747\" height=\"610\" class=\"gb-image gb-image-3554d0f9\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure2.png\" alt=\"Mitochondria-related-assays-figure2\" title=\"Mitochondria-related-assays-figure2\" srcset=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure2.png 747w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/Mitochondria-related-assays-figure2-300x245.png 300w\" sizes=\"(max-width: 747px) 100vw, 747px\" \/><\/figure>\n\n\n\n<p><strong>Figure:<\/strong>&nbsp;Effect of ionomycin and MPP+ on mitochondrial membrane depolarization by JC-1 labeling. Images indicate control cells and cells lesioned with ionomycin or MPP+ for 3 hours. In intact mitochondria, JC-1 forms red fluorescent aggregates, whereas green fluorescent monomers indicate mitochondria with depolarized membrane potential.<\/p>\n\n\n\n<p>Remark: These assays can also be customized for primary neurons.<\/p>\n\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n<h5 class=\"wp-block-heading\">Effect of Mitochondrial Toxins and Ca2+ Influx on Mitochondrial Function<\/h5>\n\n\n\n<p>Compound testing for the following indications:<\/p>\n\n\n\n<ul class=\"list__bullets\">\n<li><a href=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/services\/in-vitro-services\/parkinsons-disease-in-vitro-models\/\">Parkinson\u2018s Disease<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/services\/in-vitro-services\/alzheimers-disease-in-vitro-models\/\">Alzheimer\u2018s Disease<\/a><\/li>\n\n\n\n<li><a 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