{"id":2578,"date":"2020-02-05T13:10:03","date_gmt":"2020-02-05T13:10:03","guid":{"rendered":"https:\/\/a031212db6.nxcli.net\/in-vitro-services\/parkinsons-disease-in-vitro-models\/"},"modified":"2023-11-29T18:47:28","modified_gmt":"2023-11-30T00:47:28","slug":"parkinsons-disease-in-vitro-models","status":"publish","type":"page","link":"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/in-vitro-services\/parkinsons-disease-in-vitro-models\/","title":{"rendered":"\u30d1\u30fc\u30ad\u30f3\u30bd\u30f3\u75c5 <em>In Vitro<\/em> \u8a55\u4fa1\u7cfb"},"content":{"rendered":"<section class=\"gb-container gb-container-87da6860\">\n<div class=\"gb-container gb-container-d7ea7d2c\">\n<div class=\"gb-grid-wrapper gb-grid-wrapper-def817f0\">\n<div class=\"gb-grid-column gb-grid-column-be495a34\"><div class=\"gb-container gb-container-be495a34\">\n\n<p>Parkinson\u2019s disease (PD) is a slowly progressive neurodegenerative disease clinically characterized by progressive motor impairment in affected people. Synaptic and axonal degeneration is followed by loss of dopaminergic neurons in the substantia nigra leads to reduced levels of dopamine in the nigrostriatal circuitry. Besides dopaminergic cell loss, intracellular formation of Lewy bodies, consisting predominantly of aggregated alpha-synuclein, has been suggested to be crucial in the pathogenesis of this disease.<\/p>\n\n\n\n<p>PD is a complex, multifactorial disease in which different factors concur to the pathogenic process.&nbsp;<em>In vitro<\/em>&nbsp;models (established cell lines, primary cell cultures or lesion models) offer the advantage of a controlled environment favorable for exploring single pathogenic mechanisms and the genes\/proteins involved.<\/p>\n\n\n\n<h3 class=\"gb-headline gb-headline-b448e857 gb-headline-text\">On the cellular level, research in PD focuses on:<\/h3>\n\n\n\n<ul class=\"list__bullets\">\n<li>Neurotoxicity (MPP+, 6-OHDA, BSO,\u2026)<\/li>\n\n\n\n<li>Excitotoxicity (NMDA, glutamate,\u2026)<\/li>\n\n\n\n<li>Mitochondrial dysfunction<\/li>\n\n\n\n<li>Defects in protein degradation<\/li>\n<\/ul>\n\n\n\n<p>QPS Austria offers several cellular solutions to model PD pathology&nbsp;<em>in vitro<\/em>:<\/p>\n\n\n<div class=\"gb-container gb-container-9af486b0\">\n<div class=\"gb-container gb-container-e4f68970 gb-accordion\">\n<div class=\"gb-container gb-container-5eada5c9 gb-accordion__item\" data-transition=\"slide\">\n\n<button class=\"gb-button gb-button-e94e60dc gb-accordion__toggle\"><span class=\"gb-button-text\">\u521d\u4ee3 TH \u30cb\u30e5\u30fc\u30ed\u30f3<\/span><span class=\"gb-icon\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon-open\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><\/span><\/button>\n\n\n<div class=\"gb-accordion__content\"><div class=\"gb-container gb-container-758dca46\">\n\n<p>The degeneration of midbrain dopaminergic neurons is the hallmark of the pathology of Parkinsons Disease (PD). Dopaminergic neurons represent less than 1% of the total number of neurons in the brain, though regulating such important brain functions as motor control, motivation, and working memory. Primary cultures constitute one of the most relevant models to investigate properties and characteristics of dopaminergic neurons.<\/p>\n\n\n\n<p>QPS Austria provides primary dopaminergic TH (tyrosine hydroxylase) positive neurons from the ventral mesencephalon of rats. These cultures can be submitted to various stress agents that mimic PD pathology (MPP+, 6-OHDA) and to neuroprotective compounds in order to stop or slow down neuronal degeneration. A software supported automatic quantification method allows the determination of the following end points such as TH cell numbers, neurite outgrowth or apoptosis. A controlled regeneration or the prevention of degeneration of dopaminergic neurons are promising therapeutic approaches.<\/p>\n\n\n\n<figure class=\"gb-block-image gb-block-image-7a80ebbf\"><img loading=\"lazy\" decoding=\"async\" width=\"1047\" height=\"501\" class=\"gb-image gb-image-7a80ebbf\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/05\/TH-2.jpg\" alt=\"Number of TH positive Neurons\" title=\"TH-2\" srcset=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/05\/TH-2.jpg 1047w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/05\/TH-2-300x144.jpg 300w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/05\/TH-2-1024x490.jpg 1024w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/05\/TH-2-768x367.jpg 768w\" sizes=\"(max-width: 1047px) 100vw, 1047px\" \/><\/figure>\n\n\n\n<p><strong>Figure:<\/strong>&nbsp;Effects of a developmental Compound X on the number of primary rat TH neurons from the ventral mesencephalon.<\/p>\n\n<\/div><\/div>\n<\/div>\n\n<div class=\"gb-container gb-container-e7f02fde gb-accordion__item\" data-transition=\"slide\">\n\n<button class=\"gb-button gb-button-d9dd69de gb-accordion__toggle\"><span class=\"gb-button-text\">\u521d\u4ee3\u76ae\u8cea\u30cb\u30e5\u30fc\u30ed\u30f3\u304a\u3088\u3073\u6d77\u99ac\u30cb\u30e5\u30fc\u30ed\u30f3<\/span><span class=\"gb-icon\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon-open\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><\/span><\/button>\n\n\n<div class=\"gb-accordion__content\"><div class=\"gb-container gb-container-f56c19d6\">\n\n<p>PD involves the degeneration of nigrostriatal dopaminergic (DA) neurons. This pathology can be modeled in experimental animals by the administration of MPTP (1-metyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine), a neurotoxin which is converted in the brain into MPP+ (1-methyl-4-phenylpyridinium) by the enzyme MAO-B a toxin which selectively destroys DA neurons and causes anatomical, behavioral, and biochemical changes similar to those seen in Parkinson\u2019s disease.<\/p>\n\n\n\n<p><em>In vitro<\/em>&nbsp;Parkinsonism can be modeled by the administration of MPP+ to different cell culture systems. QPS Austria provides several cell culture models for MPP+ induced Parkinsonism, as primary cortical neurons (Figure), primary rat TH neurons, SH-SY5Y and many more.<\/p>\n\n\n\n<figure class=\"gb-block-image gb-block-image-a08f9da2\"><img loading=\"lazy\" decoding=\"async\" width=\"843\" height=\"410\" class=\"gb-image gb-image-a08f9da2\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/MPP.png\" alt=\"MPP\" title=\"MPP\" srcset=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/MPP.png 843w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/MPP-300x146.png 300w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/MPP-768x374.png 768w\" sizes=\"(max-width: 843px) 100vw, 843px\" \/><\/figure>\n\n\n\n<p><strong>Figure:<\/strong>&nbsp;Effects of known compounds as Trolox and MK-801 (left) and Compound X (right) on MPP+ induced toxicity in primary rat cortex neurons.<\/p>\n\n<\/div><\/div>\n<\/div>\n\n<div class=\"gb-container gb-container-d1fd2d24 gb-accordion__item\" data-transition=\"slide\">\n\n<button class=\"gb-button gb-button-f59bcf9a gb-accordion__toggle\"><span class=\"gb-button-text\">\u521d\u4ee3\u30d2\u30c8\u7d30\u80de<\/span><span class=\"gb-icon\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" width=\"1em\" height=\"1em\" ariahidden=\"true\" role=\"img\" class=\"gb-accordion__icon-open\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\" fill=\"currentColor\"><\/path><\/svg><\/span><\/button>\n\n\n<div class=\"gb-accordion__content\"><div class=\"gb-container gb-container-2d6e5d74\">\n\n<p>Primary fibroblasts available from patients clinically affected by:<\/p>\n\n\n\n<ul>\n<li>Parkinson Disease (PD)<\/li>\n\n\n\n<li>Leber\u2019s Hereditary Optic Neuropathy (LHON)<\/li>\n\n\n\n<li>Friedreich Ataxia (FRDA)<\/li>\n<\/ul>\n\n\n\n<p>Skin fibroblasts present a system with defined mutations and the cumulative cellular damage of the patients and can thus serve as additional model to other cell systems as primary neurons or neuroblastoma cell lines.<\/p>\n\n\n\n<p>To mimic disease related lesions of Parkinson\u2018s Disease, BSO (DL-buthionine-SR-sulfoximine) is applied for 48h to induce a toxic insult in PD cells. Cells are treated with or without Idebenone and cell viability is determined by MTT assay. Other possible toxic insults are available as MPP+, H2O2, Abeta1-42, 6-OHDA. Evaluation assays may range from cell viability to apoptosis assays or&nbsp;<a href=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/services\/in-vitro-services\/mitochondria-related-assays\/\">Mitochondrial related assays<\/a>&nbsp;(activity, distribution, shape, membrane depolarization,\u2026).<\/p>\n\n\n\n<figure class=\"gb-block-image gb-block-image-9e0231dc\"><img loading=\"lazy\" decoding=\"async\" width=\"319\" height=\"323\" class=\"gb-image gb-image-9e0231dc\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/BSO.png\" alt=\"BSO Induced Toxicity\" title=\"BSO\" srcset=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/BSO.png 319w, https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2020\/02\/BSO-296x300.png 296w\" sizes=\"(max-width: 319px) 100vw, 319px\" \/><\/figure>\n\n\n\n<p><strong>Figure:<\/strong>&nbsp;Effects of Idebenone on BSO induced neurotoxicity in human PD Fibroblasts.<\/p>\n\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div><\/div>\n\n<div class=\"gb-grid-column gb-grid-column-f501cdeb\"><div class=\"gb-container gb-container-f501cdeb\">\n<div class=\"gb-container gb-container-d1a723dd\">\n<div class=\"gb-container gb-container-2ff2e5d1\">\n\n<h3 class=\"gb-headline gb-headline-240379c5 gb-headline-text gb-headline-98ccc1d8 blueline\">Newsletter<\/h3>\n\n\n\n<div class=\"gb-grid-wrapper gb-grid-wrapper-65d3a036 gb-query-loop-wrapper\">\n<div class=\"gb-grid-column gb-grid-column-631b4a4b gb-query-loop-item post-8004 qps_newsletter type-qps_newsletter status-publish hentry\"><div class=\"gb-container gb-container-631b4a4b\">\n<div class=\"gb-headline gb-headline-93b99513 gb-headline-text\"><a href=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/qps-newsletter\/search-strategy-analysis-details-morris-water-maze-readouts\/\">Search Strategy Analysis Details Morris Water Maze Readouts<\/a><\/div>\n\n<div class=\"gb-headline gb-headline-bd23e64a gb-headline-text gb-headline-0a03f9ae\"><time class=\"entry-date published\" datetime=\"2024-01-09T08:00:24-06:00\">Jan 9, 2024<\/time><\/div>\n<\/div><\/div>\n\n<div class=\"gb-grid-column gb-grid-column-631b4a4b gb-query-loop-item post-7965 qps_newsletter type-qps_newsletter status-publish hentry\"><div class=\"gb-container gb-container-631b4a4b\">\n<div class=\"gb-headline gb-headline-93b99513 gb-headline-text\"><a href=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/qps-newsletter\/tau-phosphorylation-status-of-ps19-mice-as-model-of-tauopathies\/\">Tau Phosphorylation Status of PS19 Mice as Model of Tauopathies<\/a><\/div>\n\n<div class=\"gb-headline gb-headline-bd23e64a gb-headline-text gb-headline-0a03f9ae\"><time class=\"entry-date published\" datetime=\"2023-12-12T08:00:34-06:00\">Dec 12, 2023<\/time><\/div>\n<\/div><\/div>\n\n<div class=\"gb-grid-column gb-grid-column-631b4a4b gb-query-loop-item post-7835 qps_newsletter type-qps_newsletter 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href=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/qps-newsletter\/translational-read-out-for-preclinical-gaucher-disease-studies-assessment-of-glucosylceramidase-%ce%b2-gcase-activity-in-dried-blood-spots\/\">Translational read-out for preclinical Gaucher disease studies: Assessment of glucosylceramidase-\u03b2 (GCase) activity in dried blood spots<\/a><\/div>\n\n<div class=\"gb-headline gb-headline-bd23e64a gb-headline-text gb-headline-0a03f9ae\"><time class=\"entry-date published\" datetime=\"2023-10-09T07:00:53-05:00\">Oct 9, 2023<\/time><\/div>\n<\/div><\/div>\n\n<div class=\"gb-grid-column gb-grid-column-631b4a4b gb-query-loop-item post-7119 qps_newsletter type-qps_newsletter status-publish hentry\"><div class=\"gb-container gb-container-631b4a4b\">\n<div class=\"gb-headline gb-headline-93b99513 gb-headline-text\"><a href=\"https:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/qps-newsletter\/repeated-mptp-injections-to-model-the-parkinsons-disease-phenotype\/\">Repeated MPTP injections to model the Parkinson\u2019s disease phenotype<\/a><\/div>\n\n<div class=\"gb-headline gb-headline-bd23e64a gb-headline-text gb-headline-0a03f9ae\"><time class=\"entry-date published\" datetime=\"2023-09-18T17:54:54-05:00\">Sep 18, 2023<\/time><\/div>\n<\/div><\/div>\n<\/div>\n\n<\/div>\n\n<div class=\"gb-container gb-container-6db3cc59\">\n\n<h3 class=\"gb-headline gb-headline-43405a00 gb-headline-text gb-headline-98ccc1d8 blueline\"><em>In Vitro<\/em> Services<\/h3>\n\n\n<nav class=\"sidebar-menu wp-block-navigation is-layout-flex wp-block-navigation-is-layout-flex\" aria-label=\"In Vitro Services - Japanese\"><ul class=\"wp-block-navigation__container  sidebar-menu wp-block-navigation\"><li class=\" wp-block-navigation-item wp-block-navigation-link\"><a class=\"wp-block-navigation-item__content\"  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