{"id":7835,"date":"2023-11-02T03:49:14","date_gmt":"2023-11-02T08:49:14","guid":{"rendered":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/?post_type=qps_newsletter&#038;p=7835"},"modified":"2023-12-07T03:28:56","modified_gmt":"2023-12-07T09:28:56","slug":"repeated-electromyographic-measurements-for-longitudinal-evaluation-of-muscle-impairments","status":"publish","type":"qps_newsletter","link":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/qps-newsletter\/repeated-electromyographic-measurements-for-longitudinal-evaluation-of-muscle-impairments\/","title":{"rendered":"Repeated electromyographic measurements for longitudinal evaluation of muscle impairments"},"content":{"rendered":"\n<p>We are now able to offer longitudinal, repeated <em>in vivo<\/em> electromyographic measurements in rodent models with motor impairment. These analyses provide sensitive <em>longitudinal<\/em> readouts about your drug candidates that are unaffected by the environmental or emotional status of your animals. Typical readouts are the <strong>Compound Muscle Action Potential<\/strong> (CMAP), Motor Unit Action Potential (MUAP) and <strong>Motor Unit Number Estimation<\/strong> (MUNE).<\/p>\n\n\n\n<p>Longitudinal evaluation of the maximal CMAP amplitude in SOD1-G93A mice on a C57BL\/6J background (SOD1-B6) as model of amyotrophic lateral sclerosis resulted in progressively decreasing levels, while amplitudes of non-transgenic (ntg) animals did not change with time. CMAP amplitudes of SOD1-B6 mice were at all time points significantly reduced compared to ntg mice (Fig.1A). Evaluation of the CMAP latency to peak resulted in progressively increasing values in SOD1-B6 mice. Differences between SOD1-B6 mice and ntg littermates were significant starting at week 14 (Fig.1B).<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"507\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-1-1024x507.jpg.webp\" alt=\"Repeated electromyographic measurements\" class=\"wp-image-7836\" srcset=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-1-1024x507.jpg.webp 1024w, http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-1-1024x507.jpg-300x149.webp 300w, http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-1-1024x507.jpg-768x380.webp 768w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n\n<p><strong>Figure 1: Longitudinal CMAP measurement in anesthetized SOD1-B6 mice. <\/strong>Longitudinal measurement of the CMAP amplitude in mV (A) and longitudinal CMAP latency to peak in ms (B) in 8-, 14-, and 20-week-old SOD1-B6 mice and ntg littermates. Two-way ANOVA followed by Bonferroni&#8217;s multiple comparisons <em>post hoc <\/em>test; mean \u00b1 SEM; n = 16 per group; mixed sex; **p&lt;0.01, ***p&lt;0.001. ntg, non-transgenic.<\/p>\n\n\n\n<p>Repeated stimulation to test neuromuscular junctions\u2019 fatigue-effects resulted in significantly reduced amplitudes in SOD1-B6 mice compared to ntg littermates at all time points. Differences did not significantly change with time (Fig.2A). Longitudinal evaluation of the motor unit action potential (MUAP) in SOD1-B6 mice resulted in significantly reduced amplitudes in SOD1-B6 compared to ntg littermates at all time points. Differences did not change with time (Fig.2B).<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"425\" src=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-2-1024x425.jpg.webp\" alt=\"Repeated electromyographic measurements\" class=\"wp-image-7839\" srcset=\"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-2-1024x425.jpg.webp 1024w, http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-2-1024x425.jpg-300x125.webp 300w, http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/wp-content\/uploads\/2023\/11\/Figure-2-1024x425.jpg-768x319.webp 768w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n\n<p><strong>Figure 2: Longitudinal amplitude changes after repeated (rep.) stimulation and MUAP and measurement in anesthetized SOD1-B6 mice. <\/strong>Amplitude changes in percent after repeated stimulation (A) and longitudinal measurement of the MUAP amplitude in mV (B) in 8-, 14-, and 20-week-old SOD1-B6 mice and ntg littermates. Two-way ANOVA followed by Bonferroni&#8217;s multiple comparisons <em>post hoc <\/em>test; mean \u00b1 SEM; n = 16 per group; mixed sex; *p&lt;0.05, ***p&lt;0.001. ntg, non-transgenic.<\/p>\n\n\n\n<p><a href=\"mailto:office-austria@qps.com\">Contact us<\/a> today to get your animals tested using repeated electromyographic measurements!<\/p>\n","protected":false},"author":1,"featured_media":0,"template":"","acf":[],"_links":{"self":[{"href":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/wp-json\/wp\/v2\/qps_newsletter\/7835"}],"collection":[{"href":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/wp-json\/wp\/v2\/qps_newsletter"}],"about":[{"href":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/wp-json\/wp\/v2\/types\/qps_newsletter"}],"author":[{"embeddable":true,"href":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/wp-json\/wp\/v2\/users\/1"}],"wp:attachment":[{"href":"http:\/\/scantoxneuro.vm0857.enterprisecloud.nu\/jp\/wp-json\/wp\/v2\/media?parent=7835"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}